2008 Combined IAAM-AsTeC Workshop - November 17, 2008
Diagnostics of Invasive Aspergillosis: From Experimental Models to Clinical Evaluation
Combined IAAM and AsTeC Workshop
November 17, 2008
8:30 AM to 5:00 PM
WORKSHOP AGENDA
Click on the highlighted presenter names for presentation details
- Welcome and Greetings by NIAID (Dennis Dixon, Rory Duncan, Christine Chiou)
- AsTeC and IAAM Project overview (Tom Patterson, John Wingard)
- How these projects can contribute to the early development and clinical application of new diagnostic tests
- How these two projects are highly interactive
- What are the range and types of assays & targets that can be evaluated
- Animal Models and their Utility
- Murine model (Scott Filler and Laura Najvar)
- Rat model (Peter Warn)
- Colonization model (Don Sheppard)
- Markers of Infection - Pre-clinical Experience
- PCR evaluation (Brian Wickes and Monica Herrera)
- Beta glucan/ GM (Nathan Wiederhold)
- Host Response in Invasive Aspergillosis (Tom Walsh)
- Selected Early Diagnostic Evaluations
- Lateral Flow Device (Chris Thornton)
- Multiplex, Real-Time PCR for diagnostics (John Thornback)
- Viatrode diagnostics (Gary Fujii)
- PCR for diagnostics (Gordon Ramage)
- Aspergillus Gene Expression in Exp. Infection (Elaine Bignell – as abstract only)
- What clinical samples we will have in the AsTeC repository (John Wingard)
- Population under surveillance and sampling strategies
- Types of samples collected
- Types of clinical information recorded to annotate the samples
- What testing we can do with the AsTeC samples (Barbara Alexander)
- Participating laboratories, capabilities, and personnel
- Test evaluations:
- Reproducibility testing
- Comparative testing
- Interference substance testing
- Utilizing the AsTeC resource
- Website, IRB approvals (Lindsey Baden)
- Submission procedure (Ming-Hong Nguyen)
- Review Process (Joseph Wheat)
- Work to date
- Contamination Testing (David Denning)
- DNA extraction methods: Bead beating & lysis Buffer, Blood vs tissue samples (Angie Caliendo and Ana Vallor)
- Fungal DNA stability during storage (Ana Vallor and, David Denning)
- ISHAM Working Group: European Aspergillus PCR Initiative (J. Peter Donnelly)
- Regulatory Issues: FDA Perspective (Freddie Poole)
- Panel Discussion plus Q&A (Moderators: Tom Patterson, John Wingard)
- (Bala, Caliendo, Hillyard, Poole, Selepak, Thornback, Thornton, Verweij)
- Conclusions: Next steps, Q & A (Tom Patterson, John Wingard)
